[2018-10-12 23:31:57] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:31:57] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:31:57] Checking for Bowtie index files (genome).. [2018-10-12 23:31:57] Checking for reference FASTA file [2018-10-12 23:31:57] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:32:02] Reading known junctions from GTF file [2018-10-12 23:32:06] Preparing reads left reads: min. length=100, max. length=100, 180654 kept reads (74 discarded) right reads: min. length=100, max. length=100, 180453 kept reads (275 discarded) [2018-10-12 23:32:14] Building transcriptome data files /scratch/8792837.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:32:33] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:40:32] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:40:57] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:41:23] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:41:25] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:41:58] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:42:07] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:42:18] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:42:28] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:42:37] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:43:10] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:43:19] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:43:31] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:43:41] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:43:50] Searching for junctions via segment mapping [2018-10-12 23:46:16] Retrieving sequences for splices [2018-10-12 23:48:53] Indexing splices [2018-10-12 23:49:14] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:49:18] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:49:23] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:49:27] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:49:31] Joining segment hits [2018-10-12 23:51:46] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:51:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:51:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:51:59] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:52:02] Joining segment hits [2018-10-12 23:54:24] Reporting output tracks ----------------------------------------------- [2018-10-12 23:57:10] A summary of the alignment counts can be found in /scratch/8792837.1.linga/tophat2/align_summary.txt [2018-10-12 23:57:10] Run complete: 00:25:13 elapsed