[2018-10-13 12:48:31] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 12:48:31] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 12:48:31] Checking for Bowtie index files (genome).. [2018-10-13 12:48:31] Checking for reference FASTA file [2018-10-13 12:48:31] Generating SAM header for Bowtie2Index/genome [2018-10-13 12:48:33] Reading known junctions from GTF file [2018-10-13 12:48:35] Preparing reads left reads: min. length=100, max. length=100, 1115665 kept reads (349 discarded) right reads: min. length=100, max. length=100, 1115362 kept reads (652 discarded) [2018-10-13 12:49:09] Building transcriptome data files /scratch/8793243.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 12:49:20] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 12:54:40] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:55:32] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:56:23] Resuming TopHat pipeline with unmapped reads [2018-10-13 12:56:24] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:56:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:56:56] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:57:05] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:57:13] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:57:21] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:57:51] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:57:58] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:58:08] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:58:16] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:58:26] Searching for junctions via segment mapping [2018-10-13 13:02:26] Retrieving sequences for splices [2018-10-13 13:03:39] Indexing splices [2018-10-13 13:03:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:03:56] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:04:00] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:04:05] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:04:09] Joining segment hits [2018-10-13 13:05:32] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:05:35] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:05:40] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:05:45] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:05:50] Joining segment hits [2018-10-13 13:07:13] Reporting output tracks ----------------------------------------------- [2018-10-13 13:17:25] A summary of the alignment counts can be found in /scratch/8793243.1.c/tophat2/align_summary.txt [2018-10-13 13:17:25] Run complete: 00:28:54 elapsed