[2018-10-12 23:29:17] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:29:17] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:29:17] Checking for Bowtie index files (genome).. [2018-10-12 23:29:17] Checking for reference FASTA file [2018-10-12 23:29:17] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:29:22] Reading known junctions from GTF file [2018-10-12 23:29:26] Preparing reads left reads: min. length=100, max. length=100, 301709 kept reads (230 discarded) right reads: min. length=100, max. length=100, 301464 kept reads (475 discarded) [2018-10-12 23:29:39] Building transcriptome data files /scratch/8792836.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:29:59] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:37:53] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:38:24] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:38:56] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:38:56] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:39:33] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:39:41] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:39:53] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:40:02] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:40:10] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:40:48] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:40:57] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:41:09] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:42:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:44:16] Searching for junctions via segment mapping [2018-10-12 23:47:06] Retrieving sequences for splices [2018-10-12 23:49:17] Indexing splices [2018-10-12 23:49:36] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:49:40] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:49:44] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:49:48] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:49:52] Joining segment hits [2018-10-12 23:52:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:52:15] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:52:19] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:52:23] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:52:27] Joining segment hits [2018-10-12 23:54:37] Reporting output tracks ----------------------------------------------- [2018-10-12 23:58:02] A summary of the alignment counts can be found in /scratch/8792836.1.linga/tophat2/align_summary.txt [2018-10-12 23:58:02] Run complete: 00:28:45 elapsed