[2018-10-13 12:45:32] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 12:45:32] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 12:45:32] Checking for Bowtie index files (genome).. [2018-10-13 12:45:32] Checking for reference FASTA file [2018-10-13 12:45:32] Generating SAM header for Bowtie2Index/genome [2018-10-13 12:45:36] Reading known junctions from GTF file [2018-10-13 12:45:41] Preparing reads left reads: min. length=100, max. length=100, 1423641 kept reads (109 discarded) right reads: min. length=100, max. length=100, 1423276 kept reads (474 discarded) [2018-10-13 12:46:45] Building transcriptome data files /scratch/8793241.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 12:47:04] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 12:55:44] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:56:49] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:57:56] Resuming TopHat pipeline with unmapped reads [2018-10-13 12:57:56] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:58:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:58:51] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:59:06] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:59:19] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:59:31] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:00:22] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:00:33] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:00:50] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:01:03] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:01:17] Searching for junctions via segment mapping [2018-10-13 13:05:49] Retrieving sequences for splices [2018-10-13 13:08:06] Indexing splices [2018-10-13 13:08:27] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:08:33] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:08:41] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:08:47] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:08:53] Joining segment hits [2018-10-13 13:11:28] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:11:34] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:11:41] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:11:47] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:11:54] Joining segment hits [2018-10-13 13:14:24] Reporting output tracks ----------------------------------------------- [2018-10-13 13:23:57] A summary of the alignment counts can be found in /scratch/8793241.1.linga/tophat2/align_summary.txt [2018-10-13 13:23:57] Run complete: 00:38:25 elapsed