[2018-10-13 13:09:08] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:09:08] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:09:08] Checking for Bowtie index files (genome).. [2018-10-13 13:09:08] Checking for reference FASTA file [2018-10-13 13:09:08] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:09:12] Reading known junctions from GTF file [2018-10-13 13:09:16] Preparing reads left reads: min. length=100, max. length=100, 479350 kept reads (23 discarded) right reads: min. length=100, max. length=100, 479208 kept reads (165 discarded) [2018-10-13 13:09:41] Building transcriptome data files /scratch/8793250.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:10:00] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:18:05] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:18:36] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:19:07] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:19:07] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:19:28] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:19:36] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:19:46] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:19:54] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:20:02] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:20:22] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:20:29] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:20:39] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:20:47] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:20:56] Searching for junctions via segment mapping [2018-10-13 13:24:18] Retrieving sequences for splices [2018-10-13 13:26:17] Indexing splices [2018-10-13 13:26:38] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:26:43] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:26:48] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:26:53] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:26:58] Joining segment hits [2018-10-13 13:29:13] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:29:18] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:29:23] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:29:27] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:29:33] Joining segment hits [2018-10-13 13:31:59] Reporting output tracks ----------------------------------------------- [2018-10-13 13:38:03] A summary of the alignment counts can be found in /scratch/8793250.1.linga/tophat2/align_summary.txt [2018-10-13 13:38:03] Run complete: 00:28:54 elapsed