[2018-10-13 12:35:33] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 12:35:33] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 12:35:33] Checking for Bowtie index files (genome).. [2018-10-13 12:35:33] Checking for reference FASTA file [2018-10-13 12:35:33] Generating SAM header for Bowtie2Index/genome [2018-10-13 12:35:38] Reading known junctions from GTF file [2018-10-13 12:35:43] Preparing reads left reads: min. length=100, max. length=100, 1454540 kept reads (118 discarded) right reads: min. length=100, max. length=100, 1454156 kept reads (502 discarded) [2018-10-13 12:36:51] Building transcriptome data files /scratch/8793238.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 12:37:12] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 12:45:39] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:47:45] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:49:48] Resuming TopHat pipeline with unmapped reads [2018-10-13 12:49:48] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:50:38] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:50:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:51:07] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:51:22] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:51:35] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:52:35] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:52:49] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:53:09] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:53:26] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:53:43] Searching for junctions via segment mapping [2018-10-13 13:01:00] Retrieving sequences for splices [2018-10-13 13:03:29] Indexing splices [2018-10-13 13:03:54] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:04:02] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:04:12] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:04:22] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:04:32] Joining segment hits [2018-10-13 13:07:09] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:07:18] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:07:31] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:07:42] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:07:53] Joining segment hits [2018-10-13 13:10:37] Reporting output tracks ----------------------------------------------- [2018-10-13 13:31:22] A summary of the alignment counts can be found in /scratch/8793238.1.linga/tophat2/align_summary.txt [2018-10-13 13:31:22] Run complete: 00:55:49 elapsed