[2018-10-13 11:59:57] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 11:59:57] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 11:59:58] Checking for Bowtie index files (genome).. [2018-10-13 11:59:58] Checking for reference FASTA file [2018-10-13 11:59:58] Generating SAM header for Bowtie2Index/genome [2018-10-13 12:00:02] Reading known junctions from GTF file [2018-10-13 12:00:07] Preparing reads left reads: min. length=100, max. length=100, 2592078 kept reads (824 discarded) right reads: min. length=100, max. length=100, 2591545 kept reads (1357 discarded) [2018-10-13 12:01:48] Building transcriptome data files /scratch/8793224.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 12:02:10] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 12:10:54] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:18:29] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:26:18] Resuming TopHat pipeline with unmapped reads [2018-10-13 12:26:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:27:58] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:28:22] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:29:19] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:29:57] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:30:45] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:32:21] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:32:44] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:33:41] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:34:22] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:35:09] Searching for junctions via segment mapping [2018-10-13 13:01:48] Retrieving sequences for splices [2018-10-13 13:04:01] Indexing splices [2018-10-13 13:04:29] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:04:56] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:05:51] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:06:41] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:07:34] Joining segment hits [2018-10-13 13:10:33] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:11:01] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:11:59] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:12:52] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:13:44] Joining segment hits [2018-10-13 13:16:43] Reporting output tracks ----------------------------------------------- [2018-10-13 15:02:59] A summary of the alignment counts can be found in /scratch/8793224.1.linga/tophat2/align_summary.txt [2018-10-13 15:02:59] Run complete: 03:03:01 elapsed