[2018-10-13 17:01:25] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:01:25] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:01:26] Checking for Bowtie index files (genome).. [2018-10-13 17:01:26] Checking for reference FASTA file [2018-10-13 17:01:26] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:01:30] Reading known junctions from GTF file [2018-10-13 17:01:34] Preparing reads left reads: min. length=100, max. length=100, 773636 kept reads (515 discarded) right reads: min. length=100, max. length=100, 773087 kept reads (1064 discarded) [2018-10-13 17:02:06] Building transcriptome data files /scratch/8793381.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:02:23] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:10:56] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:11:47] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:12:39] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:12:39] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:13:19] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:13:29] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:13:44] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:13:54] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:14:05] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:14:45] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:14:56] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:15:12] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:15:24] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:15:36] Searching for junctions via segment mapping [2018-10-13 17:18:41] Retrieving sequences for splices [2018-10-13 17:20:42] Indexing splices [2018-10-13 17:21:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:21:05] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:21:10] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:21:15] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:21:19] Joining segment hits [2018-10-13 17:23:39] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:23:44] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:23:49] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:23:54] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:23:58] Joining segment hits [2018-10-13 17:26:17] Reporting output tracks ----------------------------------------------- [2018-10-13 17:30:41] A summary of the alignment counts can be found in /scratch/8793381.1.linga/tophat2/align_summary.txt [2018-10-13 17:30:41] Run complete: 00:29:15 elapsed