[2018-10-13 11:46:24] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 11:46:24] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 11:46:24] Checking for Bowtie index files (genome).. [2018-10-13 11:46:24] Checking for reference FASTA file [2018-10-13 11:46:24] Generating SAM header for Bowtie2Index/genome [2018-10-13 11:46:29] Reading known junctions from GTF file [2018-10-13 11:46:34] Preparing reads left reads: min. length=100, max. length=100, 2622455 kept reads (507 discarded) right reads: min. length=100, max. length=100, 2621787 kept reads (1175 discarded) [2018-10-13 11:48:26] Building transcriptome data files /scratch/8793215.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 11:48:48] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 11:57:09] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:06:44] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:16:33] Resuming TopHat pipeline with unmapped reads [2018-10-13 12:16:33] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:18:01] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:18:27] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:19:28] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:20:09] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:21:01] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:22:32] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:22:51] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:23:48] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:24:28] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:25:13] Searching for junctions via segment mapping [2018-10-13 12:55:51] Retrieving sequences for splices [2018-10-13 12:58:15] Indexing splices [2018-10-13 12:58:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 12:59:27] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:00:28] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:01:17] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:02:14] Joining segment hits [2018-10-13 13:05:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:05:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:06:51] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:07:42] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:08:35] Joining segment hits [2018-10-13 13:11:34] Reporting output tracks ----------------------------------------------- [2018-10-13 15:23:52] A summary of the alignment counts can be found in /scratch/8793215.1.linga/tophat2/align_summary.txt [2018-10-13 15:23:52] Run complete: 03:37:27 elapsed