[2018-10-13 11:46:28] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 11:46:28] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 11:46:28] Checking for Bowtie index files (genome).. [2018-10-13 11:46:28] Checking for reference FASTA file [2018-10-13 11:46:28] Generating SAM header for Bowtie2Index/genome [2018-10-13 11:46:32] Reading known junctions from GTF file [2018-10-13 11:46:37] Preparing reads left reads: min. length=100, max. length=100, 1139164 kept reads (73 discarded) right reads: min. length=100, max. length=100, 1138967 kept reads (270 discarded) [2018-10-13 11:47:25] Building transcriptome data files /scratch/8793214.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 11:47:44] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 11:55:49] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 11:57:59] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 12:00:12] Resuming TopHat pipeline with unmapped reads [2018-10-13 12:00:12] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:01:02] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:01:14] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:01:36] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:01:49] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:02:03] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 12:02:50] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 12:03:02] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 12:03:25] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 12:03:42] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 12:03:58] Searching for junctions via segment mapping [2018-10-13 12:13:19] Retrieving sequences for splices [2018-10-13 12:15:31] Indexing splices [2018-10-13 12:16:02] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 12:16:14] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 12:16:28] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 12:16:42] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 12:16:54] Joining segment hits [2018-10-13 12:19:30] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 12:19:40] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 12:19:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 12:20:06] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 12:20:19] Joining segment hits [2018-10-13 12:22:58] Reporting output tracks ----------------------------------------------- [2018-10-13 12:51:56] A summary of the alignment counts can be found in /scratch/8793214.1.linga/tophat2/align_summary.txt [2018-10-13 12:51:56] Run complete: 01:05:28 elapsed