[2018-10-12 23:15:16] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:15:16] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:15:16] Checking for Bowtie index files (genome).. [2018-10-12 23:15:16] Checking for reference FASTA file [2018-10-12 23:15:16] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:15:20] Reading known junctions from GTF file [2018-10-12 23:15:25] Preparing reads left reads: min. length=100, max. length=100, 302437 kept reads (225 discarded) right reads: min. length=100, max. length=100, 302162 kept reads (500 discarded) [2018-10-12 23:15:38] Building transcriptome data files /scratch/8792831.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:15:58] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:25:03] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:25:35] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:26:07] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:26:07] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:26:43] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:26:52] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:27:04] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:27:13] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:27:23] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:28:00] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:28:10] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:28:22] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:28:32] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:28:43] Searching for junctions via segment mapping [2018-10-12 23:31:23] Retrieving sequences for splices [2018-10-12 23:33:33] Indexing splices [2018-10-12 23:33:55] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:34:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:34:05] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:34:09] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:34:13] Joining segment hits [2018-10-12 23:36:44] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:36:49] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:36:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:36:58] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:37:02] Joining segment hits [2018-10-12 23:39:29] Reporting output tracks ----------------------------------------------- [2018-10-12 23:43:54] A summary of the alignment counts can be found in /scratch/8792831.1.linga/tophat2/align_summary.txt [2018-10-12 23:43:54] Run complete: 00:28:37 elapsed