[2018-10-12 23:12:40] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:12:40] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:12:40] Checking for Bowtie index files (genome).. [2018-10-12 23:12:40] Checking for reference FASTA file [2018-10-12 23:12:40] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:12:43] Reading known junctions from GTF file [2018-10-12 23:12:45] Preparing reads left reads: min. length=100, max. length=100, 635005 kept reads (349 discarded) right reads: min. length=100, max. length=100, 634621 kept reads (733 discarded) [2018-10-12 23:13:04] Building transcriptome data files /scratch/8792829.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:13:16] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:18:35] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:19:12] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:19:49] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:19:49] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:20:26] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:20:31] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:20:39] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:20:45] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:20:51] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:21:28] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:21:34] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:21:42] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:21:48] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:21:55] Searching for junctions via segment mapping [2018-10-12 23:23:30] Retrieving sequences for splices [2018-10-12 23:24:43] Indexing splices [2018-10-12 23:24:55] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:24:58] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:25:00] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:25:03] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:25:05] Joining segment hits [2018-10-12 23:26:23] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:26:25] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:26:28] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:26:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:26:33] Joining segment hits [2018-10-12 23:27:57] Reporting output tracks ----------------------------------------------- [2018-10-12 23:30:10] A summary of the alignment counts can be found in /scratch/8792829.1.c/tophat2/align_summary.txt [2018-10-12 23:30:10] Run complete: 00:17:29 elapsed