[2018-10-12 23:12:35] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:12:35] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:12:35] Checking for Bowtie index files (genome).. [2018-10-12 23:12:35] Checking for reference FASTA file [2018-10-12 23:12:35] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:12:42] Reading known junctions from GTF file [2018-10-12 23:12:49] Preparing reads left reads: min. length=100, max. length=100, 453646 kept reads (273 discarded) right reads: min. length=100, max. length=100, 453293 kept reads (626 discarded) [2018-10-12 23:13:17] Building transcriptome data files /scratch/8792828.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:13:39] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:24:28] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:25:27] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:26:29] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:26:29] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:27:39] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:27:53] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:28:10] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:28:23] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:28:35] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:29:45] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:30:01] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:30:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:30:44] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:31:01] Searching for junctions via segment mapping [2018-10-12 23:34:36] Retrieving sequences for splices [2018-10-12 23:37:29] Indexing splices [2018-10-12 23:37:58] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:38:06] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:38:14] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:38:21] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:38:28] Joining segment hits [2018-10-12 23:42:19] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:42:26] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:43:21] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:44:05] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:44:12] Joining segment hits [2018-10-12 23:47:06] Reporting output tracks ----------------------------------------------- [2018-10-12 23:51:30] A summary of the alignment counts can be found in /scratch/8792828.1.linga/tophat2/align_summary.txt [2018-10-12 23:51:30] Run complete: 00:38:54 elapsed