[2018-10-13 18:07:42] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:07:42] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:07:42] Checking for Bowtie index files (genome).. [2018-10-13 18:07:42] Checking for reference FASTA file [2018-10-13 18:07:42] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:07:44] Reading known junctions from GTF file [2018-10-13 18:07:46] Preparing reads left reads: min. length=100, max. length=100, 374411 kept reads (258 discarded) right reads: min. length=100, max. length=100, 374055 kept reads (614 discarded) [2018-10-13 18:07:57] Building transcriptome data files /scratch/8793428.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:08:08] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:12:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:13:09] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:13:28] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:13:28] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:13:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:13:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:13:55] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:13:59] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:14:04] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:14:21] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:14:26] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:14:32] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:14:37] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:14:42] Searching for junctions via segment mapping [2018-10-13 18:16:02] Retrieving sequences for splices [2018-10-13 18:17:09] Indexing splices [2018-10-13 18:17:21] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:17:23] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:17:25] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:17:27] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:17:30] Joining segment hits [2018-10-13 18:18:40] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:18:42] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:18:45] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:18:47] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:18:49] Joining segment hits [2018-10-13 18:20:05] Reporting output tracks ----------------------------------------------- [2018-10-13 18:21:44] A summary of the alignment counts can be found in /scratch/8793428.1.p16/tophat2/align_summary.txt [2018-10-13 18:21:44] Run complete: 00:14:01 elapsed