[2018-10-12 23:06:48] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:06:48] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:06:48] Checking for Bowtie index files (genome).. [2018-10-12 23:06:48] Checking for reference FASTA file [2018-10-12 23:06:48] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:06:53] Reading known junctions from GTF file [2018-10-12 23:06:57] Preparing reads left reads: min. length=100, max. length=100, 393081 kept reads (220 discarded) right reads: min. length=100, max. length=100, 392729 kept reads (572 discarded) [2018-10-12 23:07:14] Building transcriptome data files /scratch/8792824.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:07:35] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:16:18] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:16:54] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:17:31] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:17:31] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:18:14] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:18:24] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:18:37] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:18:47] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:18:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:19:42] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:19:53] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:20:06] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:20:18] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:20:29] Searching for junctions via segment mapping [2018-10-12 23:23:11] Retrieving sequences for splices [2018-10-12 23:25:21] Indexing splices [2018-10-12 23:25:42] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:25:46] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:25:51] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:25:55] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:26:00] Joining segment hits [2018-10-12 23:28:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:28:32] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:28:37] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:28:41] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:28:45] Joining segment hits [2018-10-12 23:31:16] Reporting output tracks ----------------------------------------------- [2018-10-12 23:34:50] A summary of the alignment counts can be found in /scratch/8792824.1.linga/tophat2/align_summary.txt [2018-10-12 23:34:50] Run complete: 00:28:02 elapsed