[2018-10-13 11:06:05] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 11:06:05] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 11:06:05] Checking for Bowtie index files (genome).. [2018-10-13 11:06:05] Checking for reference FASTA file [2018-10-13 11:06:05] Generating SAM header for Bowtie2Index/genome [2018-10-13 11:06:11] Reading known junctions from GTF file [2018-10-13 11:06:15] Preparing reads left reads: min. length=100, max. length=100, 653857 kept reads (234 discarded) right reads: min. length=100, max. length=100, 653576 kept reads (515 discarded) [2018-10-13 11:06:48] Building transcriptome data files /scratch/8793194.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 11:07:09] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 11:15:59] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 11:16:49] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 11:17:43] Resuming TopHat pipeline with unmapped reads [2018-10-13 11:17:43] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 11:18:16] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 11:18:26] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 11:18:39] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 11:18:50] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 11:19:01] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 11:19:37] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 11:19:46] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 11:20:00] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 11:20:11] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 11:20:22] Searching for junctions via segment mapping [2018-10-13 11:24:34] Retrieving sequences for splices [2018-10-13 11:26:52] Indexing splices [2018-10-13 11:27:15] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 11:27:19] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 11:27:25] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 11:27:30] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 11:27:35] Joining segment hits [2018-10-13 11:30:04] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 11:30:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 11:30:15] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 11:30:20] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 11:30:25] Joining segment hits [2018-10-13 11:33:05] Reporting output tracks ----------------------------------------------- [2018-10-13 11:39:24] A summary of the alignment counts can be found in /scratch/8793194.1.linga/tophat2/align_summary.txt [2018-10-13 11:39:24] Run complete: 00:33:18 elapsed