[2018-10-13 11:03:34] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 11:03:34] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 11:03:34] Checking for Bowtie index files (genome).. [2018-10-13 11:03:34] Checking for reference FASTA file [2018-10-13 11:03:34] Generating SAM header for Bowtie2Index/genome [2018-10-13 11:03:36] Reading known junctions from GTF file [2018-10-13 11:03:38] Preparing reads left reads: min. length=100, max. length=100, 666544 kept reads (92 discarded) right reads: min. length=100, max. length=100, 666242 kept reads (394 discarded) [2018-10-13 11:03:57] Building transcriptome data files /scratch/8793192.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 11:04:07] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 11:09:40] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 11:10:05] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 11:10:30] Resuming TopHat pipeline with unmapped reads [2018-10-13 11:10:30] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 11:10:48] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 11:10:53] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 11:10:59] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 11:11:04] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 11:11:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 11:11:27] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 11:11:31] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 11:11:38] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 11:11:43] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 11:11:48] Searching for junctions via segment mapping [2018-10-13 11:13:35] Retrieving sequences for splices [2018-10-13 11:14:47] Indexing splices [2018-10-13 11:14:59] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 11:15:01] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 11:15:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 11:15:07] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 11:15:09] Joining segment hits [2018-10-13 11:16:30] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 11:16:32] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 11:16:35] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 11:16:38] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 11:16:40] Joining segment hits [2018-10-13 11:18:01] Reporting output tracks ----------------------------------------------- [2018-10-13 11:20:39] A summary of the alignment counts can be found in /scratch/8793192.1.c/tophat2/align_summary.txt [2018-10-13 11:20:39] Run complete: 00:17:04 elapsed