[2018-10-13 10:51:29] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 10:51:29] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 10:51:29] Checking for Bowtie index files (genome).. [2018-10-13 10:51:29] Checking for reference FASTA file [2018-10-13 10:51:29] Generating SAM header for Bowtie2Index/genome [2018-10-13 10:51:31] Reading known junctions from GTF file [2018-10-13 10:51:33] Preparing reads left reads: min. length=100, max. length=100, 937280 kept reads (150 discarded) right reads: min. length=100, max. length=100, 936946 kept reads (484 discarded) [2018-10-13 10:52:00] Building transcriptome data files /scratch/8793188.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 10:52:13] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:56:55] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:57:35] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:58:13] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:58:13] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:58:31] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:58:36] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:58:42] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:58:48] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:58:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:59:13] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:59:18] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:59:25] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:59:31] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:59:37] Searching for junctions via segment mapping [2018-10-13 11:02:19] Retrieving sequences for splices [2018-10-13 11:03:26] Indexing splices [2018-10-13 11:03:39] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 11:03:42] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 11:03:45] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 11:03:49] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 11:03:52] Joining segment hits [2018-10-13 11:05:08] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 11:05:11] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 11:05:14] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 11:05:18] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 11:05:22] Joining segment hits [2018-10-13 11:06:37] Reporting output tracks ----------------------------------------------- [2018-10-13 11:14:37] A summary of the alignment counts can be found in /scratch/8793188.1.p8/tophat2/align_summary.txt [2018-10-13 11:14:37] Run complete: 00:23:07 elapsed