[2018-10-12 23:06:47] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 23:06:47] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 23:06:47] Checking for Bowtie index files (genome).. [2018-10-12 23:06:47] Checking for reference FASTA file [2018-10-12 23:06:47] Generating SAM header for Bowtie2Index/genome [2018-10-12 23:06:51] Reading known junctions from GTF file [2018-10-12 23:06:56] Preparing reads left reads: min. length=100, max. length=100, 3315196 kept reads (278 discarded) right reads: min. length=100, max. length=100, 3314186 kept reads (1288 discarded) [2018-10-12 23:09:10] Building transcriptome data files /scratch/8792822.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 23:09:29] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:18:07] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:20:05] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:22:05] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:22:05] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:23:28] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:23:43] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:24:13] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:24:31] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:24:49] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:26:16] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:26:33] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:27:05] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:27:26] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:27:46] Searching for junctions via segment mapping [2018-10-12 23:33:41] Retrieving sequences for splices [2018-10-12 23:36:06] Indexing splices [2018-10-12 23:36:28] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:36:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:36:46] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:36:55] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:37:04] Joining segment hits [2018-10-12 23:39:58] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:40:06] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:40:16] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:40:25] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:40:33] Joining segment hits [2018-10-12 23:43:27] Reporting output tracks ----------------------------------------------- [2018-10-13 00:02:32] A summary of the alignment counts can be found in /scratch/8792822.1.linga/tophat2/align_summary.txt [2018-10-13 00:02:32] Run complete: 00:55:45 elapsed