[2018-10-13 18:04:15] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:04:15] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:04:15] Checking for Bowtie index files (genome).. [2018-10-13 18:04:15] Checking for reference FASTA file [2018-10-13 18:04:15] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:04:19] Reading known junctions from GTF file [2018-10-13 18:04:24] Preparing reads left reads: min. length=100, max. length=100, 358278 kept reads (208 discarded) right reads: min. length=100, max. length=100, 357943 kept reads (543 discarded) [2018-10-13 18:04:39] Building transcriptome data files /scratch/8793426.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:05:00] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:13:21] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:13:54] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:14:27] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:14:27] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:14:57] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:15:06] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:15:18] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:15:28] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:15:38] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:16:09] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:16:18] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:16:31] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:16:41] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:16:51] Searching for junctions via segment mapping [2018-10-13 18:19:33] Retrieving sequences for splices [2018-10-13 18:21:39] Indexing splices [2018-10-13 18:21:59] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:22:03] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:22:08] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:22:13] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:22:17] Joining segment hits [2018-10-13 18:24:40] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:24:44] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:24:49] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:24:54] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:24:58] Joining segment hits [2018-10-13 18:27:24] Reporting output tracks ----------------------------------------------- [2018-10-13 18:30:47] A summary of the alignment counts can be found in /scratch/8793426.1.linga/tophat2/align_summary.txt [2018-10-13 18:30:47] Run complete: 00:26:32 elapsed