[2018-10-13 10:14:22] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 10:14:22] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 10:14:22] Checking for Bowtie index files (genome).. [2018-10-13 10:14:22] Checking for reference FASTA file [2018-10-13 10:14:22] Generating SAM header for Bowtie2Index/genome [2018-10-13 10:14:24] Reading known junctions from GTF file [2018-10-13 10:14:27] Preparing reads left reads: min. length=100, max. length=100, 1289213 kept reads (115 discarded) right reads: min. length=100, max. length=100, 1288834 kept reads (494 discarded) [2018-10-13 10:15:02] Building transcriptome data files /scratch/8793170.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 10:15:12] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:19:54] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:20:31] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:21:07] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:21:08] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:21:31] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:21:37] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:21:44] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:21:51] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:21:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:22:22] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:22:28] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:22:36] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:22:43] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:22:50] Searching for junctions via segment mapping [2018-10-13 10:25:02] Retrieving sequences for splices [2018-10-13 10:26:09] Indexing splices [2018-10-13 10:26:21] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:26:23] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:26:26] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:26:29] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:26:32] Joining segment hits [2018-10-13 10:27:49] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:27:51] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:27:54] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:27:57] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:28:00] Joining segment hits [2018-10-13 10:29:16] Reporting output tracks ----------------------------------------------- [2018-10-13 10:34:35] A summary of the alignment counts can be found in /scratch/8793170.1.p16/tophat2/align_summary.txt [2018-10-13 10:34:35] Run complete: 00:20:13 elapsed