[2018-10-13 10:14:27] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 10:14:27] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 10:14:28] Checking for Bowtie index files (genome).. [2018-10-13 10:14:28] Checking for reference FASTA file [2018-10-13 10:14:28] Generating SAM header for Bowtie2Index/genome [2018-10-13 10:14:31] Reading known junctions from GTF file [2018-10-13 10:14:35] Preparing reads left reads: min. length=100, max. length=100, 1168142 kept reads (115 discarded) right reads: min. length=100, max. length=100, 1167901 kept reads (356 discarded) [2018-10-13 10:15:25] Building transcriptome data files /scratch/8793168.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 10:15:43] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:23:45] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:24:54] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:26:04] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:26:05] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:26:49] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:27:01] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:27:19] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:27:32] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:27:44] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:28:29] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:28:40] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:28:59] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:29:13] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:29:26] Searching for junctions via segment mapping [2018-10-13 10:35:41] Retrieving sequences for splices [2018-10-13 10:37:41] Indexing splices [2018-10-13 10:38:03] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:38:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:38:23] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:38:32] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:38:40] Joining segment hits [2018-10-13 10:41:22] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:41:30] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:41:42] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:41:51] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:42:00] Joining segment hits [2018-10-13 10:44:22] Reporting output tracks ----------------------------------------------- [2018-10-13 10:58:57] A summary of the alignment counts can be found in /scratch/8793168.1.linga/tophat2/align_summary.txt [2018-10-13 10:58:57] Run complete: 00:44:30 elapsed