[2018-10-13 10:04:16] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 10:04:16] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 10:04:16] Checking for Bowtie index files (genome).. [2018-10-13 10:04:16] Checking for reference FASTA file [2018-10-13 10:04:16] Generating SAM header for Bowtie2Index/genome [2018-10-13 10:04:18] Reading known junctions from GTF file [2018-10-13 10:04:21] Preparing reads left reads: min. length=100, max. length=100, 992964 kept reads (155 discarded) right reads: min. length=100, max. length=100, 992688 kept reads (431 discarded) [2018-10-13 10:04:46] Building transcriptome data files /scratch/8793167.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 10:04:56] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:09:38] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:10:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:10:42] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:10:42] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:11:03] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:11:08] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:11:14] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:11:19] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:11:24] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:11:47] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:11:52] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:11:59] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:12:05] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:12:10] Searching for junctions via segment mapping [2018-10-13 10:14:10] Retrieving sequences for splices [2018-10-13 10:15:17] Indexing splices [2018-10-13 10:15:29] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:15:32] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:15:35] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:15:37] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:15:40] Joining segment hits [2018-10-13 10:16:56] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:16:58] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:17:01] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:17:04] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:17:07] Joining segment hits [2018-10-13 10:18:23] Reporting output tracks ----------------------------------------------- [2018-10-13 10:23:25] A summary of the alignment counts can be found in /scratch/8793167.1.p8/tophat2/align_summary.txt [2018-10-13 10:23:25] Run complete: 00:19:08 elapsed