[2018-10-13 10:04:07] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 10:04:07] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 10:04:07] Checking for Bowtie index files (genome).. [2018-10-13 10:04:07] Checking for reference FASTA file [2018-10-13 10:04:07] Generating SAM header for Bowtie2Index/genome [2018-10-13 10:04:11] Reading known junctions from GTF file [2018-10-13 10:04:15] Preparing reads left reads: min. length=100, max. length=100, 1622023 kept reads (96 discarded) right reads: min. length=100, max. length=100, 1621338 kept reads (781 discarded) [2018-10-13 10:05:24] Building transcriptome data files /scratch/8793166.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 10:05:42] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:13:43] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:14:56] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:16:11] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:16:11] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:16:46] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:16:55] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:17:08] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:17:20] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:17:30] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:18:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:18:22] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:18:36] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:18:49] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:19:01] Searching for junctions via segment mapping [2018-10-13 10:23:14] Retrieving sequences for splices [2018-10-13 10:25:17] Indexing splices [2018-10-13 10:25:41] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:25:49] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:25:56] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:26:03] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:26:09] Joining segment hits [2018-10-13 10:28:44] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:28:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:28:57] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:29:04] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:29:11] Joining segment hits [2018-10-13 10:31:40] Reporting output tracks ----------------------------------------------- [2018-10-13 10:45:21] A summary of the alignment counts can be found in /scratch/8793166.1.linga/tophat2/align_summary.txt [2018-10-13 10:45:21] Run complete: 00:41:14 elapsed