[2018-10-13 18:03:23] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:03:23] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:03:23] Checking for Bowtie index files (genome).. [2018-10-13 18:03:23] Checking for reference FASTA file [2018-10-13 18:03:23] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:03:27] Reading known junctions from GTF file [2018-10-13 18:03:32] Preparing reads left reads: min. length=100, max. length=100, 463266 kept reads (188 discarded) right reads: min. length=100, max. length=100, 462999 kept reads (455 discarded) [2018-10-13 18:03:53] Building transcriptome data files /scratch/8793425.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:04:12] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:12:28] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:13:04] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:13:41] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:13:42] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:14:14] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:14:23] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:14:36] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:14:46] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:14:56] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:15:31] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:15:41] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:15:54] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:16:05] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:16:15] Searching for junctions via segment mapping [2018-10-13 18:19:12] Retrieving sequences for splices [2018-10-13 18:21:24] Indexing splices [2018-10-13 18:21:45] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:21:50] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:21:55] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:22:00] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:22:04] Joining segment hits [2018-10-13 18:24:28] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:24:32] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:24:38] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:24:43] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:24:47] Joining segment hits [2018-10-13 18:27:10] Reporting output tracks ----------------------------------------------- [2018-10-13 18:31:00] A summary of the alignment counts can be found in /scratch/8793425.1.linga/tophat2/align_summary.txt [2018-10-13 18:31:00] Run complete: 00:27:37 elapsed