[2018-10-12 22:46:42] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:46:42] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:46:42] Checking for Bowtie index files (genome).. [2018-10-12 22:46:42] Checking for reference FASTA file [2018-10-12 22:46:42] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:46:44] Reading known junctions from GTF file [2018-10-12 22:46:47] Preparing reads left reads: min. length=100, max. length=100, 360223 kept reads (182 discarded) right reads: min. length=100, max. length=100, 359754 kept reads (651 discarded) [2018-10-12 22:46:56] Building transcriptome data files /scratch/8792810.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:47:06] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:51:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:52:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:52:30] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:52:30] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:52:50] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:52:55] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:53:00] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:53:05] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:53:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:53:30] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:53:35] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:53:41] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:53:46] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:53:51] Searching for junctions via segment mapping [2018-10-12 22:55:10] Retrieving sequences for splices [2018-10-12 22:56:17] Indexing splices [2018-10-12 22:56:29] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:56:31] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:56:34] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:56:36] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:56:38] Joining segment hits [2018-10-12 22:57:54] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:57:56] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:57:58] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:58:01] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:58:03] Joining segment hits [2018-10-12 22:59:18] Reporting output tracks ----------------------------------------------- [2018-10-12 23:01:01] A summary of the alignment counts can be found in /scratch/8792810.1.p16/tophat2/align_summary.txt [2018-10-12 23:01:01] Run complete: 00:14:18 elapsed