[2018-10-12 22:46:42] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:46:42] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:46:42] Checking for Bowtie index files (genome).. [2018-10-12 22:46:42] Checking for reference FASTA file [2018-10-12 22:46:42] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:46:44] Reading known junctions from GTF file [2018-10-12 22:46:46] Preparing reads left reads: min. length=100, max. length=100, 478794 kept reads (186 discarded) right reads: min. length=100, max. length=100, 478501 kept reads (479 discarded) [2018-10-12 22:47:01] Building transcriptome data files /scratch/8792809.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:47:11] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:51:54] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:52:19] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:52:45] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:52:45] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:53:12] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:53:17] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:53:22] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:53:27] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:53:31] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:53:59] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:54:04] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:54:09] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:54:15] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:54:19] Searching for junctions via segment mapping [2018-10-12 22:55:43] Retrieving sequences for splices [2018-10-12 22:56:49] Indexing splices [2018-10-12 22:57:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:57:02] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:57:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:57:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:57:09] Joining segment hits [2018-10-12 22:58:25] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:58:27] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:58:29] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:58:32] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:58:34] Joining segment hits [2018-10-12 22:59:51] Reporting output tracks ----------------------------------------------- [2018-10-12 23:01:58] A summary of the alignment counts can be found in /scratch/8792809.1.p8/tophat2/align_summary.txt [2018-10-12 23:01:58] Run complete: 00:15:15 elapsed