[2018-10-13 16:59:41] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 16:59:41] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 16:59:41] Checking for Bowtie index files (genome).. [2018-10-13 16:59:41] Checking for reference FASTA file [2018-10-13 16:59:41] Generating SAM header for Bowtie2Index/genome [2018-10-13 16:59:45] Reading known junctions from GTF file [2018-10-13 16:59:49] Preparing reads left reads: min. length=100, max. length=100, 276575 kept reads (280 discarded) right reads: min. length=100, max. length=100, 276359 kept reads (496 discarded) [2018-10-13 17:00:01] Building transcriptome data files /scratch/8793378.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:00:20] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:08:24] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:08:55] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:09:24] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:09:24] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:09:56] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:10:05] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:10:17] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:10:26] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:10:35] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:11:08] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:11:18] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:11:31] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:11:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:11:50] Searching for junctions via segment mapping [2018-10-13 17:14:23] Retrieving sequences for splices [2018-10-13 17:16:37] Indexing splices [2018-10-13 17:16:56] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:17:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:17:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:17:08] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:17:12] Joining segment hits [2018-10-13 17:19:30] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:19:34] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:19:39] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:19:43] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:19:47] Joining segment hits [2018-10-13 17:22:07] Reporting output tracks ----------------------------------------------- [2018-10-13 17:25:14] A summary of the alignment counts can be found in /scratch/8793378.1.linga/tophat2/align_summary.txt [2018-10-13 17:25:14] Run complete: 00:25:32 elapsed