[2018-10-13 09:46:40] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 09:46:40] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 09:46:40] Checking for Bowtie index files (genome).. [2018-10-13 09:46:40] Checking for reference FASTA file [2018-10-13 09:46:40] Generating SAM header for Bowtie2Index/genome [2018-10-13 09:46:43] Reading known junctions from GTF file [2018-10-13 09:46:45] Preparing reads left reads: min. length=100, max. length=100, 1551687 kept reads (121 discarded) right reads: min. length=100, max. length=100, 1550896 kept reads (912 discarded) [2018-10-13 09:47:30] Building transcriptome data files /scratch/8793155.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 09:47:40] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 09:52:23] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:53:04] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:53:47] Resuming TopHat pipeline with unmapped reads [2018-10-13 09:53:47] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:54:11] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:54:16] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:54:23] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:54:29] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:54:35] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:55:03] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:55:10] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:55:18] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:55:25] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:55:32] Searching for junctions via segment mapping [2018-10-13 09:57:49] Retrieving sequences for splices [2018-10-13 09:58:56] Indexing splices [2018-10-13 09:59:08] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:59:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:59:14] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:59:18] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:59:21] Joining segment hits [2018-10-13 10:00:38] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:00:41] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:00:44] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:00:48] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:00:51] Joining segment hits [2018-10-13 10:02:09] Reporting output tracks ----------------------------------------------- [2018-10-13 10:09:25] A summary of the alignment counts can be found in /scratch/8793155.1.p16/tophat2/align_summary.txt [2018-10-13 10:09:25] Run complete: 00:22:44 elapsed