[2018-10-13 09:58:21] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 09:58:21] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 09:58:21] Checking for Bowtie index files (genome).. [2018-10-13 09:58:21] Checking for reference FASTA file [2018-10-13 09:58:21] Generating SAM header for Bowtie2Index/genome [2018-10-13 09:58:25] Reading known junctions from GTF file [2018-10-13 09:58:29] Preparing reads left reads: min. length=100, max. length=100, 1267246 kept reads (115 discarded) right reads: min. length=100, max. length=100, 1266760 kept reads (601 discarded) [2018-10-13 09:59:17] Building transcriptome data files /scratch/8793161.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 09:59:34] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:06:42] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:07:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:08:33] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:08:33] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:09:03] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:09:11] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:09:23] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:09:34] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:09:44] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:10:17] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:10:26] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:10:39] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:10:51] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:11:03] Searching for junctions via segment mapping [2018-10-13 10:15:08] Retrieving sequences for splices [2018-10-13 10:17:09] Indexing splices [2018-10-13 10:17:30] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:17:35] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:17:42] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:17:48] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:17:54] Joining segment hits [2018-10-13 10:20:36] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:20:42] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:20:50] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:20:56] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:21:03] Joining segment hits [2018-10-13 10:23:24] Reporting output tracks ----------------------------------------------- [2018-10-13 10:33:08] A summary of the alignment counts can be found in /scratch/8793161.1.linga/tophat2/align_summary.txt [2018-10-13 10:33:08] Run complete: 00:34:47 elapsed