[2018-10-13 09:58:21] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 09:58:21] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 09:58:21] Checking for Bowtie index files (genome).. [2018-10-13 09:58:21] Checking for reference FASTA file [2018-10-13 09:58:21] Generating SAM header for Bowtie2Index/genome [2018-10-13 09:58:25] Reading known junctions from GTF file [2018-10-13 09:58:30] Preparing reads left reads: min. length=100, max. length=100, 1131343 kept reads (109 discarded) right reads: min. length=100, max. length=100, 1131050 kept reads (402 discarded) [2018-10-13 09:59:14] Building transcriptome data files /scratch/8793160.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 09:59:33] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 10:07:54] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:09:20] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 10:10:48] Resuming TopHat pipeline with unmapped reads [2018-10-13 10:10:48] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:11:30] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:11:41] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:12:00] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:12:12] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:12:25] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 10:13:10] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 10:13:21] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 10:13:39] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 10:13:52] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 10:14:05] Searching for junctions via segment mapping [2018-10-13 10:20:58] Retrieving sequences for splices [2018-10-13 10:23:28] Indexing splices [2018-10-13 10:23:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:24:01] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:24:12] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:24:21] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:24:31] Joining segment hits [2018-10-13 10:27:01] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 10:27:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 10:27:20] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 10:27:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 10:27:39] Joining segment hits [2018-10-13 10:30:38] Reporting output tracks ----------------------------------------------- [2018-10-13 10:51:26] A summary of the alignment counts can be found in /scratch/8793160.1.linga/tophat2/align_summary.txt [2018-10-13 10:51:26] Run complete: 00:53:04 elapsed