[2018-10-12 22:52:30] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:52:30] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:52:30] Checking for Bowtie index files (genome).. [2018-10-12 22:52:30] Checking for reference FASTA file [2018-10-12 22:52:30] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:52:34] Reading known junctions from GTF file [2018-10-12 22:52:38] Preparing reads left reads: min. length=100, max. length=100, 154784 kept reads (110 discarded) right reads: min. length=100, max. length=100, 154629 kept reads (265 discarded) [2018-10-12 22:52:45] Building transcriptome data files /scratch/8792813.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:53:05] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 23:01:07] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:01:26] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 23:01:46] Resuming TopHat pipeline with unmapped reads [2018-10-12 23:01:46] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:02:08] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:02:16] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:02:25] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:02:34] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:02:42] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 23:03:04] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 23:03:12] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 23:03:22] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 23:03:31] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 23:03:39] Searching for junctions via segment mapping [2018-10-12 23:06:02] Retrieving sequences for splices [2018-10-12 23:08:12] Indexing splices [2018-10-12 23:08:32] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:08:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:08:40] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:08:44] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:08:48] Joining segment hits [2018-10-12 23:11:09] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:11:13] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:11:17] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:11:21] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:11:25] Joining segment hits [2018-10-12 23:13:39] Reporting output tracks ----------------------------------------------- [2018-10-12 23:16:41] A summary of the alignment counts can be found in /scratch/8792813.1.linga/tophat2/align_summary.txt [2018-10-12 23:16:41] Run complete: 00:24:11 elapsed