[2018-10-13 09:32:11] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 09:32:11] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 09:32:11] Checking for Bowtie index files (genome).. [2018-10-13 09:32:11] Checking for reference FASTA file [2018-10-13 09:32:11] Generating SAM header for Bowtie2Index/genome [2018-10-13 09:32:15] Reading known junctions from GTF file [2018-10-13 09:32:20] Preparing reads left reads: min. length=100, max. length=100, 119793 kept reads (107 discarded) right reads: min. length=100, max. length=100, 119685 kept reads (215 discarded) [2018-10-13 09:32:25] Building transcriptome data files /scratch/8793148.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 09:32:44] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 09:40:00] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:40:15] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:40:30] Resuming TopHat pipeline with unmapped reads [2018-10-13 09:40:30] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:40:47] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:40:55] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:41:04] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:41:12] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:41:20] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:41:37] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:41:45] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:41:56] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:42:04] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:42:12] Searching for junctions via segment mapping [2018-10-13 09:44:22] Retrieving sequences for splices [2018-10-13 09:46:29] Indexing splices [2018-10-13 09:46:47] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:46:51] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:46:55] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:46:59] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:47:03] Joining segment hits [2018-10-13 09:49:15] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:49:18] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:49:23] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:49:26] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:49:31] Joining segment hits [2018-10-13 09:51:43] Reporting output tracks ----------------------------------------------- [2018-10-13 09:54:05] A summary of the alignment counts can be found in /scratch/8793148.1.linga/tophat2/align_summary.txt [2018-10-13 09:54:05] Run complete: 00:21:54 elapsed