[2018-10-13 09:18:57] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 09:18:57] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 09:18:57] Checking for Bowtie index files (genome).. [2018-10-13 09:18:57] Checking for reference FASTA file [2018-10-13 09:18:57] Generating SAM header for Bowtie2Index/genome [2018-10-13 09:19:02] Reading known junctions from GTF file [2018-10-13 09:19:06] Preparing reads left reads: min. length=100, max. length=100, 838167 kept reads (508 discarded) right reads: min. length=100, max. length=100, 837940 kept reads (735 discarded) [2018-10-13 09:19:37] Building transcriptome data files /scratch/8793143.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 09:19:58] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 09:28:34] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:30:32] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:32:32] Resuming TopHat pipeline with unmapped reads [2018-10-13 09:32:32] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:33:20] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:33:33] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:33:58] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:34:15] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:34:30] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:35:20] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:35:37] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:36:03] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:36:20] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:36:36] Searching for junctions via segment mapping [2018-10-13 09:46:43] Retrieving sequences for splices [2018-10-13 09:49:01] Indexing splices [2018-10-13 09:49:27] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:49:39] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:49:57] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:50:12] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:50:24] Joining segment hits [2018-10-13 09:52:46] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:52:58] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:53:16] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:53:32] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:53:44] Joining segment hits [2018-10-13 09:56:09] Reporting output tracks ----------------------------------------------- [2018-10-13 10:25:01] A summary of the alignment counts can be found in /scratch/8793143.1.linga/tophat2/align_summary.txt [2018-10-13 10:25:01] Run complete: 01:06:03 elapsed