[2018-10-12 22:38:27] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:38:27] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:38:27] Checking for Bowtie index files (genome).. [2018-10-12 22:38:27] Checking for reference FASTA file [2018-10-12 22:38:27] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:38:31] Reading known junctions from GTF file [2018-10-12 22:38:36] Preparing reads left reads: min. length=100, max. length=100, 445386 kept reads (315 discarded) right reads: min. length=100, max. length=100, 445118 kept reads (583 discarded) [2018-10-12 22:38:56] Building transcriptome data files /scratch/8792806.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:39:18] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:48:31] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:49:17] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:50:03] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:50:03] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:50:50] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:51:00] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:51:13] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:51:24] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:51:35] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:52:24] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:52:35] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:52:49] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:53:01] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:53:12] Searching for junctions via segment mapping [2018-10-12 22:56:05] Retrieving sequences for splices [2018-10-12 22:58:21] Indexing splices [2018-10-12 22:58:42] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:58:46] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:58:51] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:58:56] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:59:00] Joining segment hits [2018-10-12 23:01:34] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:01:43] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:01:48] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:01:52] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:01:57] Joining segment hits [2018-10-12 23:04:31] Reporting output tracks ----------------------------------------------- [2018-10-12 23:08:22] A summary of the alignment counts can be found in /scratch/8792806.1.linga/tophat2/align_summary.txt [2018-10-12 23:08:22] Run complete: 00:29:54 elapsed