[2018-10-12 22:38:28] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:38:28] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:38:28] Checking for Bowtie index files (genome).. [2018-10-12 22:38:28] Checking for reference FASTA file [2018-10-12 22:38:28] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:38:33] Reading known junctions from GTF file [2018-10-12 22:38:38] Preparing reads left reads: min. length=100, max. length=100, 443816 kept reads (279 discarded) right reads: min. length=100, max. length=100, 443489 kept reads (606 discarded) [2018-10-12 22:38:57] Building transcriptome data files /scratch/8792805.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:39:17] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:48:23] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:49:04] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:49:46] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:49:46] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:50:26] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:50:35] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:50:48] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:50:59] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:51:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:51:50] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:52:01] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:52:15] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:52:26] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:52:38] Searching for junctions via segment mapping [2018-10-12 22:55:27] Retrieving sequences for splices [2018-10-12 22:57:39] Indexing splices [2018-10-12 22:58:02] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:58:06] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:58:11] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:58:15] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:58:19] Joining segment hits [2018-10-12 23:00:51] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 23:00:55] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 23:01:00] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 23:01:05] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 23:01:09] Joining segment hits [2018-10-12 23:03:42] Reporting output tracks ----------------------------------------------- [2018-10-12 23:07:22] A summary of the alignment counts can be found in /scratch/8792805.1.linga/tophat2/align_summary.txt [2018-10-12 23:07:22] Run complete: 00:28:53 elapsed