[2018-10-13 09:05:55] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 09:05:55] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 09:05:55] Checking for Bowtie index files (genome).. [2018-10-13 09:05:55] Checking for reference FASTA file [2018-10-13 09:05:55] Generating SAM header for Bowtie2Index/genome [2018-10-13 09:06:00] Reading known junctions from GTF file [2018-10-13 09:06:04] Preparing reads left reads: min. length=100, max. length=100, 960782 kept reads (146 discarded) right reads: min. length=100, max. length=100, 960538 kept reads (390 discarded) [2018-10-13 09:06:47] Building transcriptome data files /scratch/8793134.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 09:07:06] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 09:15:45] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:16:32] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 09:17:19] Resuming TopHat pipeline with unmapped reads [2018-10-13 09:17:19] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:17:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:18:00] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:18:13] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:18:23] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:18:34] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 09:19:08] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 09:19:18] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 09:19:32] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 09:19:44] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 09:19:55] Searching for junctions via segment mapping [2018-10-13 09:23:49] Retrieving sequences for splices [2018-10-13 09:25:58] Indexing splices [2018-10-13 09:26:22] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:26:27] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:26:33] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:26:40] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:26:46] Joining segment hits [2018-10-13 09:29:14] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:29:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:29:25] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:29:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:29:36] Joining segment hits [2018-10-13 09:32:20] Reporting output tracks ----------------------------------------------- [2018-10-13 09:40:00] A summary of the alignment counts can be found in /scratch/8793134.1.linga/tophat2/align_summary.txt [2018-10-13 09:40:00] Run complete: 00:34:05 elapsed