[2018-10-13 08:40:20] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 08:40:20] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 08:40:20] Checking for Bowtie index files (genome).. [2018-10-13 08:40:20] Checking for reference FASTA file [2018-10-13 08:40:20] Generating SAM header for Bowtie2Index/genome [2018-10-13 08:40:25] Reading known junctions from GTF file [2018-10-13 08:40:29] Preparing reads left reads: min. length=100, max. length=100, 355208 kept reads (228 discarded) right reads: min. length=100, max. length=100, 354834 kept reads (602 discarded) [2018-10-13 08:40:45] Building transcriptome data files /scratch/8793122.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 08:41:04] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 08:49:38] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:50:16] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:50:54] Resuming TopHat pipeline with unmapped reads [2018-10-13 08:50:54] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:51:34] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:51:44] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:51:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:52:08] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:52:18] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:52:59] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:53:09] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:53:23] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:53:35] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:53:46] Searching for junctions via segment mapping [2018-10-13 08:56:25] Retrieving sequences for splices [2018-10-13 08:58:33] Indexing splices [2018-10-13 08:58:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:58:57] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:59:02] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:59:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:59:10] Joining segment hits [2018-10-13 09:01:41] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 09:01:46] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 09:01:50] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 09:01:55] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 09:01:59] Joining segment hits [2018-10-13 09:04:38] Reporting output tracks ----------------------------------------------- [2018-10-13 09:08:07] A summary of the alignment counts can be found in /scratch/8793122.1.linga/tophat2/align_summary.txt [2018-10-13 09:08:07] Run complete: 00:27:46 elapsed