[2018-10-13 08:15:32] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 08:15:32] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 08:15:32] Checking for Bowtie index files (genome).. [2018-10-13 08:15:32] Checking for reference FASTA file [2018-10-13 08:15:32] Generating SAM header for Bowtie2Index/genome [2018-10-13 08:15:37] Reading known junctions from GTF file [2018-10-13 08:15:42] Preparing reads left reads: min. length=100, max. length=100, 1310718 kept reads (90 discarded) right reads: min. length=100, max. length=100, 1310236 kept reads (572 discarded) [2018-10-13 08:16:43] Building transcriptome data files /scratch/8793109.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 08:17:05] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 08:25:32] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:26:54] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:28:17] Resuming TopHat pipeline with unmapped reads [2018-10-13 08:28:17] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:28:56] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:29:06] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:29:21] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:29:33] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:29:45] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:30:27] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:30:38] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:30:53] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:31:05] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:31:18] Searching for junctions via segment mapping [2018-10-13 08:36:24] Retrieving sequences for splices [2018-10-13 08:38:34] Indexing splices [2018-10-13 08:38:59] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:39:06] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:39:14] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:39:22] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:39:29] Joining segment hits [2018-10-13 08:42:00] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:42:07] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:42:15] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:42:23] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:42:31] Joining segment hits [2018-10-13 08:45:02] Reporting output tracks ----------------------------------------------- [2018-10-13 09:01:28] A summary of the alignment counts can be found in /scratch/8793109.1.linga/tophat2/align_summary.txt [2018-10-13 09:01:28] Run complete: 00:45:55 elapsed