[2018-10-13 18:01:41] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:01:41] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:01:41] Checking for Bowtie index files (genome).. [2018-10-13 18:01:41] Checking for reference FASTA file [2018-10-13 18:01:41] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:01:45] Reading known junctions from GTF file [2018-10-13 18:01:51] Preparing reads left reads: min. length=100, max. length=100, 481472 kept reads (281 discarded) right reads: min. length=100, max. length=100, 481144 kept reads (609 discarded) [2018-10-13 18:02:17] Building transcriptome data files /scratch/8793422.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:02:42] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:11:37] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:12:26] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:13:16] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:13:16] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:14:01] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:14:11] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:14:25] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:14:37] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:14:48] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:15:32] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:15:44] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:15:59] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:16:11] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:16:22] Searching for junctions via segment mapping [2018-10-13 18:19:21] Retrieving sequences for splices [2018-10-13 18:21:46] Indexing splices [2018-10-13 18:22:08] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:22:13] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:22:19] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:22:24] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:22:28] Joining segment hits [2018-10-13 18:25:04] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:25:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:25:15] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:25:20] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:25:25] Joining segment hits [2018-10-13 18:28:04] Reporting output tracks ----------------------------------------------- [2018-10-13 18:32:05] A summary of the alignment counts can be found in /scratch/8793422.1.linga/tophat2/align_summary.txt [2018-10-13 18:32:05] Run complete: 00:30:24 elapsed