[2018-10-13 08:06:07] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 08:06:07] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 08:06:07] Checking for Bowtie index files (genome).. [2018-10-13 08:06:07] Checking for reference FASTA file [2018-10-13 08:06:07] Generating SAM header for Bowtie2Index/genome [2018-10-13 08:06:11] Reading known junctions from GTF file [2018-10-13 08:06:15] Preparing reads left reads: min. length=100, max. length=100, 892394 kept reads (81 discarded) right reads: min. length=100, max. length=100, 892105 kept reads (370 discarded) [2018-10-13 08:06:49] Building transcriptome data files /scratch/8793106.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 08:07:06] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 08:15:13] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:15:55] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:16:35] Resuming TopHat pipeline with unmapped reads [2018-10-13 08:16:35] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:17:09] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:17:20] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:17:33] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:17:43] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:17:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:18:23] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:18:32] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:18:44] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:18:54] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:19:05] Searching for junctions via segment mapping [2018-10-13 08:22:37] Retrieving sequences for splices [2018-10-13 08:24:45] Indexing splices [2018-10-13 08:25:07] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:25:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:25:17] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:25:22] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:25:26] Joining segment hits [2018-10-13 08:27:55] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:27:59] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:28:05] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:28:09] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:28:14] Joining segment hits [2018-10-13 08:30:48] Reporting output tracks ----------------------------------------------- [2018-10-13 08:36:16] A summary of the alignment counts can be found in /scratch/8793106.1.linga/tophat2/align_summary.txt [2018-10-13 08:36:16] Run complete: 00:30:09 elapsed