[2018-10-12 22:32:41] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 22:32:41] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 22:32:41] Checking for Bowtie index files (genome).. [2018-10-12 22:32:41] Checking for reference FASTA file [2018-10-12 22:32:41] Generating SAM header for Bowtie2Index/genome [2018-10-12 22:32:46] Reading known junctions from GTF file [2018-10-12 22:32:50] Preparing reads left reads: min. length=100, max. length=100, 495383 kept reads (243 discarded) right reads: min. length=100, max. length=100, 495023 kept reads (603 discarded) [2018-10-12 22:33:13] Building transcriptome data files /scratch/8792798.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 22:33:31] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 22:41:49] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:42:33] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 22:43:18] Resuming TopHat pipeline with unmapped reads [2018-10-12 22:43:18] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:44:06] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:44:16] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:44:31] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:44:42] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:44:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 22:45:41] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 22:45:52] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 22:46:07] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 22:46:19] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 22:46:31] Searching for junctions via segment mapping [2018-10-12 22:49:20] Retrieving sequences for splices [2018-10-12 22:51:28] Indexing splices [2018-10-12 22:51:51] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:51:56] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:52:01] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:52:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:52:11] Joining segment hits [2018-10-12 22:54:43] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 22:54:48] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 22:54:53] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 22:54:58] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 22:55:03] Joining segment hits [2018-10-12 22:57:34] Reporting output tracks ----------------------------------------------- [2018-10-12 23:01:45] A summary of the alignment counts can be found in /scratch/8792798.1.linga/tophat2/align_summary.txt [2018-10-12 23:01:45] Run complete: 00:29:04 elapsed