[2018-10-13 08:33:07] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 08:33:07] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 08:33:07] Checking for Bowtie index files (genome).. [2018-10-13 08:33:07] Checking for reference FASTA file [2018-10-13 08:33:07] Generating SAM header for Bowtie2Index/genome [2018-10-13 08:33:12] Reading known junctions from GTF file [2018-10-13 08:33:15] Preparing reads left reads: min. length=100, max. length=100, 1395816 kept reads (68 discarded) right reads: min. length=100, max. length=100, 1395261 kept reads (623 discarded) [2018-10-13 08:34:09] Building transcriptome data files /scratch/8793120.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 08:34:28] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 08:42:19] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:43:28] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:44:40] Resuming TopHat pipeline with unmapped reads [2018-10-13 08:44:40] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:45:09] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:45:18] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:45:30] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:45:40] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:45:51] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:46:27] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:46:37] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:46:50] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:47:02] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:47:14] Searching for junctions via segment mapping [2018-10-13 08:51:43] Retrieving sequences for splices [2018-10-13 08:53:46] Indexing splices [2018-10-13 08:54:08] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:54:15] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:54:21] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:54:28] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:54:34] Joining segment hits [2018-10-13 08:56:56] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:57:02] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:57:09] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:57:16] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:57:23] Joining segment hits [2018-10-13 08:59:48] Reporting output tracks ----------------------------------------------- [2018-10-13 09:12:46] A summary of the alignment counts can be found in /scratch/8793120.1.linga/tophat2/align_summary.txt [2018-10-13 09:12:46] Run complete: 00:39:38 elapsed