[2018-10-13 07:57:05] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:57:05] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:57:05] Checking for Bowtie index files (genome).. [2018-10-13 07:57:05] Checking for reference FASTA file [2018-10-13 07:57:05] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:57:09] Reading known junctions from GTF file [2018-10-13 07:57:14] Preparing reads left reads: min. length=100, max. length=100, 960866 kept reads (174 discarded) right reads: min. length=100, max. length=100, 960570 kept reads (470 discarded) [2018-10-13 07:57:54] Building transcriptome data files /scratch/8793100.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:58:14] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 08:06:23] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:07:41] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 08:09:00] Resuming TopHat pipeline with unmapped reads [2018-10-13 08:09:00] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:09:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:09:57] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:10:16] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:10:29] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:10:41] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 08:11:28] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 08:11:40] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 08:12:01] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:12:14] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:12:28] Searching for junctions via segment mapping [2018-10-13 08:20:00] Retrieving sequences for splices [2018-10-13 08:22:09] Indexing splices [2018-10-13 08:22:36] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:22:45] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:22:57] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:23:06] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:23:14] Joining segment hits [2018-10-13 08:26:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:26:38] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:26:51] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:27:02] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:27:12] Joining segment hits [2018-10-13 08:30:00] Reporting output tracks ----------------------------------------------- [2018-10-13 08:48:30] A summary of the alignment counts can be found in /scratch/8793100.1.linga/tophat2/align_summary.txt [2018-10-13 08:48:30] Run complete: 00:51:25 elapsed