[2018-10-13 07:44:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:44:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:44:04] Checking for Bowtie index files (genome).. [2018-10-13 07:44:04] Checking for reference FASTA file [2018-10-13 07:44:04] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:44:08] Reading known junctions from GTF file [2018-10-13 07:44:12] Preparing reads left reads: min. length=100, max. length=100, 833229 kept reads (658 discarded) right reads: min. length=100, max. length=100, 833020 kept reads (867 discarded) [2018-10-13 07:44:50] Building transcriptome data files /scratch/8793093.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:45:10] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:54:02] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:55:16] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:56:24] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:56:24] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:57:15] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:57:25] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:57:49] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:58:03] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:58:18] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:59:15] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:59:25] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:59:51] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 08:00:05] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 08:00:21] Searching for junctions via segment mapping [2018-10-13 08:06:26] Retrieving sequences for splices [2018-10-13 08:08:38] Indexing splices [2018-10-13 08:09:01] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:09:06] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:09:16] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:09:24] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:09:33] Joining segment hits [2018-10-13 08:12:02] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 08:12:08] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 08:12:17] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 08:12:28] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:12:38] Joining segment hits [2018-10-13 08:15:08] Reporting output tracks ----------------------------------------------- [2018-10-13 08:29:01] A summary of the alignment counts can be found in /scratch/8793093.1.linga/tophat2/align_summary.txt [2018-10-13 08:29:01] Run complete: 00:44:57 elapsed