[2018-10-13 07:37:00] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:37:00] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:37:00] Checking for Bowtie index files (genome).. [2018-10-13 07:37:00] Checking for reference FASTA file [2018-10-13 07:37:00] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:37:02] Reading known junctions from GTF file [2018-10-13 07:37:04] Preparing reads left reads: min. length=100, max. length=100, 674362 kept reads (97 discarded) right reads: min. length=100, max. length=100, 674173 kept reads (286 discarded) [2018-10-13 07:37:24] Building transcriptome data files /scratch/8793091.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:37:34] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:42:15] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:42:43] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:43:11] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:43:11] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:43:27] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:43:31] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:43:36] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:43:41] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:43:46] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:44:02] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:44:06] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:44:12] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:44:17] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:44:22] Searching for junctions via segment mapping [2018-10-13 07:46:32] Retrieving sequences for splices [2018-10-13 07:47:40] Indexing splices [2018-10-13 07:47:53] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:47:55] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:47:58] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:48:01] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:48:04] Joining segment hits [2018-10-13 07:49:19] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:49:22] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:49:25] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:49:28] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:49:31] Joining segment hits [2018-10-13 07:50:46] Reporting output tracks ----------------------------------------------- [2018-10-13 07:56:07] A summary of the alignment counts can be found in /scratch/8793091.1.p16/tophat2/align_summary.txt [2018-10-13 07:56:07] Run complete: 00:19:07 elapsed