[2018-10-13 07:37:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:37:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:37:04] Checking for Bowtie index files (genome).. [2018-10-13 07:37:04] Checking for reference FASTA file [2018-10-13 07:37:04] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:37:07] Reading known junctions from GTF file [2018-10-13 07:37:11] Preparing reads left reads: min. length=100, max. length=100, 1241551 kept reads (132 discarded) right reads: min. length=100, max. length=100, 1241094 kept reads (589 discarded) [2018-10-13 07:37:59] Building transcriptome data files /scratch/8793090.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:38:17] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:45:38] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:46:34] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:47:30] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:47:30] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:48:08] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:48:17] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:48:32] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:48:43] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:48:54] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:49:34] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:49:44] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:50:00] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:50:12] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:50:24] Searching for junctions via segment mapping [2018-10-13 07:54:11] Retrieving sequences for splices [2018-10-13 07:56:21] Indexing splices [2018-10-13 07:56:42] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:56:48] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:56:54] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:57:00] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:57:05] Joining segment hits [2018-10-13 07:59:38] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:59:44] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:59:50] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:59:56] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 08:00:02] Joining segment hits [2018-10-13 08:02:47] Reporting output tracks ----------------------------------------------- [2018-10-13 08:11:25] A summary of the alignment counts can be found in /scratch/8793090.1.linga/tophat2/align_summary.txt [2018-10-13 08:11:25] Run complete: 00:34:21 elapsed