[2018-10-13 17:59:51] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:59:51] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:59:51] Checking for Bowtie index files (genome).. [2018-10-13 17:59:51] Checking for reference FASTA file [2018-10-13 17:59:51] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:59:53] Reading known junctions from GTF file [2018-10-13 17:59:56] Preparing reads left reads: min. length=100, max. length=100, 281617 kept reads (145 discarded) right reads: min. length=100, max. length=100, 281332 kept reads (430 discarded) [2018-10-13 18:00:04] Building transcriptome data files /scratch/8793421.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:00:13] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:04:56] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:05:11] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:05:27] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:05:27] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:05:42] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:05:47] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:05:52] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:05:56] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:06:00] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:06:15] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:06:20] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:06:25] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:06:29] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:06:34] Searching for junctions via segment mapping [2018-10-13 18:07:52] Retrieving sequences for splices [2018-10-13 18:08:58] Indexing splices [2018-10-13 18:09:09] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:09:11] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:09:14] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:09:16] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:09:18] Joining segment hits [2018-10-13 18:10:33] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:10:35] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:10:38] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:10:40] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:10:42] Joining segment hits [2018-10-13 18:11:56] Reporting output tracks ----------------------------------------------- [2018-10-13 18:13:33] A summary of the alignment counts can be found in /scratch/8793421.1.p16/tophat2/align_summary.txt [2018-10-13 18:13:33] Run complete: 00:13:41 elapsed