[2018-10-13 07:23:43] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:23:43] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:23:43] Checking for Bowtie index files (genome).. [2018-10-13 07:23:43] Checking for reference FASTA file [2018-10-13 07:23:43] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:23:47] Reading known junctions from GTF file [2018-10-13 07:23:51] Preparing reads left reads: min. length=100, max. length=100, 1270757 kept reads (97 discarded) right reads: min. length=100, max. length=100, 1270460 kept reads (394 discarded) [2018-10-13 07:24:44] Building transcriptome data files /scratch/8793085.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:25:04] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:33:05] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:34:05] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:35:05] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:35:05] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:35:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:35:50] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:36:03] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:36:14] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:36:24] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:37:02] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:37:11] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:37:24] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:37:35] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:37:46] Searching for junctions via segment mapping [2018-10-13 07:41:39] Retrieving sequences for splices [2018-10-13 07:43:51] Indexing splices [2018-10-13 07:44:12] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:44:17] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:44:24] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:44:29] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:44:35] Joining segment hits [2018-10-13 07:47:04] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:47:10] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:47:16] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:47:22] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:47:28] Joining segment hits [2018-10-13 07:50:01] Reporting output tracks ----------------------------------------------- [2018-10-13 07:59:35] A summary of the alignment counts can be found in /scratch/8793085.1.linga/tophat2/align_summary.txt [2018-10-13 07:59:35] Run complete: 00:35:51 elapsed