[2018-10-13 07:20:25] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 07:20:25] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 07:20:25] Checking for Bowtie index files (genome).. [2018-10-13 07:20:25] Checking for reference FASTA file [2018-10-13 07:20:25] Generating SAM header for Bowtie2Index/genome [2018-10-13 07:20:29] Reading known junctions from GTF file [2018-10-13 07:20:33] Preparing reads left reads: min. length=100, max. length=100, 1232489 kept reads (348 discarded) right reads: min. length=100, max. length=100, 1232178 kept reads (659 discarded) [2018-10-13 07:21:22] Building transcriptome data files /scratch/8793082.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 07:21:42] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 07:30:09] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:32:23] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 07:34:38] Resuming TopHat pipeline with unmapped reads [2018-10-13 07:34:38] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:35:23] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:35:33] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:35:55] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:36:10] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:36:26] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 07:37:11] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 07:37:21] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 07:37:43] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 07:37:59] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 07:38:15] Searching for junctions via segment mapping [2018-10-13 07:48:31] Retrieving sequences for splices [2018-10-13 07:50:35] Indexing splices [2018-10-13 07:50:57] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:51:05] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:51:18] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:51:31] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:51:43] Joining segment hits [2018-10-13 07:54:09] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 07:54:17] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 07:54:31] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 07:54:44] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 07:54:57] Joining segment hits [2018-10-13 07:57:26] Reporting output tracks ----------------------------------------------- [2018-10-13 08:27:16] A summary of the alignment counts can be found in /scratch/8793082.1.linga/tophat2/align_summary.txt [2018-10-13 08:27:16] Run complete: 01:06:51 elapsed